The mechanism of hypogammaglobuinemia caused by ketongenic diet
10.3760/cma.j.issn.0254-5101.2014.11.002
- VernacularTitle:生酮饮食对难治性癫痫患儿滤泡辅助性T细胞及B细胞的影响
- Author:
Houfu ZHOU
;
Chengrong LI
;
Jianxiang LIAO
;
Guobing WANG
;
Sufang LIN
;
Li CHEN
- Publication Type:Journal Article
- Keywords:
Ketogenic diet;
Mammalian target of rapamycin;
B-lymphocytes;
Follicular helper T cells;
IL-21;
Bcl-6;
Blimp-1
- From:Chinese Journal of Microbiology and Immunology
2014;(11):817-824
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism of hypogammaglobuinemia caused by ketongenic diet (KD).Methods Thirty-six children with intractable epilepsy (IP) and seventeen age-matched healthy children were recruited in this study.The percentages of B cells at various stages of devel-opment and follicular helper T ( Tfh) cells were detected by flow cytometry.The plasma concentrations of IL-21 were determined by ELISA.Real-time quantitative PCR was performed to detect the expression of mam-malian target of rapamycin ( mTOR) , Blimp-1, Bcl-6 and IL-21 at mRNA level in CD4+T cells.Results mTOR at mRNA level was significantly down-regulated after KD treatment (P<0.05).The numbers of Tfh cells were positively correlated with the transcriptional level of mTOR (r=-0.691, P<0.05).Conclusion KD treatment might down-regulate Tfh and B cells through suppressing the expression of mTOR at mRNA level, suggesting a possible mechanism of hypogammaglobuinemia induced by KD treatment.
