Application of high resolution melting analysis in the rapid identification of functional class 2 inte-gron
10.3760/cma.j.issn.0254-5101.2014.11.007
- VernacularTitle:应用高分辨熔解曲线法快速鉴定功能性第2类整合子
- Author:
Wanxiang LI
;
Quhao WEI
;
Qingfeng HU
;
Sufeng CHEN
;
Xiaowei LIN
;
Huoyang LYU
- Publication Type:Journal Article
- Keywords:
Integron;
High resolution melting analysis;
Integrase
- From:
Chinese Journal of Microbiology and Immunology
2014;(11):849-851
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high resolution melting based method for the rapid identifica-tion of functional class 2 integron.Methods Nighty-nine non-repetitive Proteus spp.strains positive for genes encoding class 2 integrase were isolated from August, 2011 to August, 2012.The genomic DNAs were extracted and used as templates to amplify 60 base pair fragments containing the mutated point in class 2 in-tegrase gene by PCR.The high resolution melting analysis was conducted to identify the functional class 2 in-tegrons that were further compared by sequence analysis.Results There were remarkable differences with the high resolution melting curves between the functional class 2 integrons and the ordinary class 2 integrons. The results of high resolution melting analysis were consistent with those by using sequence analysis.Con-clusion High resolution melting analysis could be used for the rapid and accurate identification of functional class 2 integron.