Application of high resolution melting analysis in the rapid identification of functional class 2 inte-gron

VernacularTitle:应用高分辨熔解曲线法快速鉴定功能性第2类整合子
Author: Wanxiang LI ; Quhao WEI ; Qingfeng HU ; Sufeng CHEN ; Xiaowei LIN ; Huoyang LYU
Publication Type:Journal Article
Keywords: Integron; High resolution melting analysis; Integrase
From: Chinese Journal of Microbiology and Immunology 2014;(11):849-851
CountryChina
Language:Chinese
Abstract: Objective To establish a high resolution melting based method for the rapid identifica-tion of functional class 2 integron.Methods Nighty-nine non-repetitive Proteus spp.strains positive for genes encoding class 2 integrase were isolated from August, 2011 to August, 2012.The genomic DNAs were extracted and used as templates to amplify 60 base pair fragments containing the mutated point in class 2 in-tegrase gene by PCR.The high resolution melting analysis was conducted to identify the functional class 2 in-tegrons that were further compared by sequence analysis.Results There were remarkable differences with the high resolution melting curves between the functional class 2 integrons and the ordinary class 2 integrons. The results of high resolution melting analysis were consistent with those by using sequence analysis.Con-clusion High resolution melting analysis could be used for the rapid and accurate identification of functional class 2 integron.