Determination of Teicoplanin in Human Cerebrospinal Fluid Using an On-line Solid Phase Extraction-Two Dimensional Liquid Chromatography-Tandem Mass Spactrometry
10.11895/j.issn.0253-3820.140758
- VernacularTitle:在线固相萃取-二维液相色谱串联质谱法测定人脑脊液中替考拉宁浓度
- Author:
Limin ZUO
;
Jing YAO
;
Qiang WANG
;
Jie ZHOU
;
Mengxue FENG
;
Guangzhi SHAN
- Publication Type:Journal Article
- Keywords:
On-line solid phase extraction;
Teicoplanin;
Cerebrospinal fluid;
Two-dimensional liquid chromatography-tandem mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2014;(12):1773-1778
- CountryChina
- Language:Chinese
-
Abstract:
A method of on-line solid phase extraction ( SPE )-two dimensional liquid chromatography electrospary-tandem mass spectrometric method was established for the determination of Teicoplanin concentrations in human cerebrospinal fluid. Cerebrospinal fluid samples were treated by the on-line SPE treatment, and analyzed by LC-MS/MS. The chromatographic separation was performed on a Shiseido CAPCALL-PAK C18 column with gradient elution by using 25 mmol/L ammonium acetate ( pH 6. 0 )-acetonitrile as mobile phases, and the flow rate of 1 mL/min. Detection was carried out under the selected reaction monitoring ( SRM) in positive ionization mode with scopolamine hydrobromide as internal standard. Matrix-matched calibration curves with good correlation coefficients (R2=0. 9993, n=6) were obtained in the concentration range of 25-5000 μg/L. The average recoveries varied from 100. 8% to 109. 9%. The intra-and inter-day precisions were less than 6%. The method is proved to be rapid, sensitive, accurate, and suitable to determine Teicoplanin concentrations in human cerebrospinal fluid.
