Determination of Astragaloside IV in Radix Astragli and Its Preparations by Online SPE-Liquid Chromatography with Charged Aerosol Detector
10.11895/j.issn.0253-3820.140767
- VernacularTitle:在线固相萃取法结合电雾式检测器测定黄芪及其复方中黄芪甲苷的含量
- Author:
Xiaokuan LI
;
Yanhai ZHANG
;
Tianhui FENG
;
Yanling YANG
;
Yan JIN
- Publication Type:Journal Article
- Keywords:
Astragaloside IV,Online solid phase extraction;
Corona chard aerosol detection;
Dual liquid chromatography
- From:
Chinese Journal of Analytical Chemistry
2014;(12):1791-1796
- CountryChina
- Language:Chinese
-
Abstract:
A novel method for quantification of astragaloside IV in Radix Astragli was developed by using online SPE liquid chromatography coupled with corona charged aerosol detection( CAD) . The sample solution
was loaded into Acclaim Polar AdvantageⅡC18(50 mm×4. 6 mm, 3 μm) which was selected as online SPE column. Then the cleaning process was done by using the Right one of dual gradient pumps with Methanol-water as mobile phase. Acclaim C18(150 mm×4. 6 mm, 5 μm) was selected as analytical column with acetonitrile-water as mobile phase at a flow rate of 1. 0 mL/min. The eluate containing the target from SPE column was transferred into the analytical column by heart-cutting mode. The temperature of nebulizer of corona CAD was set at 30 ℃ and the nitrogen pressure was 241. 3 kPa. The baseline separation of astragaloside IV from matrix components has been achieved. There was a good linear correlation in the range of 4 . 0-80 mg/L for astragaloside IV and the correlation coefficient was 0 . 9998 . The standard addition average recovery of astragaloside IV in Radix Astragli was 97 . 6%. It has been validated that astragaloside IV in Radix Astragli and its preparations can be quantified rapidly and accurately with this method.
