Fully Automated Determination of Four Androgenic Hormones in Serum by Online Turbulent Flow Solid Phase Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.140726
- VernacularTitle:全自动在线涡流固相萃取-液相色谱-串联质谱法直接分析血清中4种雄激素
- Author:
Feng GUO
;
Jianbo SHI
;
Guibin JIANG
- Publication Type:Journal Article
- Keywords:
Androgenic hormones;
Serum;
Online solid phase extraction;
Liquid chromatography;
Mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2014;(12):1818-1822
- CountryChina
- Language:Chinese
-
Abstract:
A novel method was developed for the direct analysis of testosterone, androstenedione, methyltestosterone and methenolone in serum samples by fully automated online turbulent flow solid phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry. An aliquot of 50 μL serum sample was preconcentrated directly on a Turboflow SPE column after centrifugation. Turboflow SPE C18-P could be used to remove serum matrix effectively. The optimum loading flow rate and elution time were 4. 0 mL/min and 1. 0 min, respectively. The linearity ranges were from 1. 0 μg/L to 100. 0 μg/L for four target compounds. The method limits of detection ( LODs) were in the range of 0. 2-0. 3 μg/L. The relative standard deviations (RSDs) ranged from 2. 9% to 14. 1% (n=5). The time for one sample analysis including extraction, separation and determination was 32 min. The proposed method has been successfully applied for the analysis of serum samples.
