Determination of 2460 A in Trichoderma Hazianum Fermentation Liquor by On-line Two-dimensional Liquid Chromatographic Method
10.11895/j.issn.0253-3820.140755
- VernacularTitle:二维液相色谱法在线检测哈茨木霉发酵液中2460 A含量
- Author:
Guangzhi SHAN
;
Jie ZHOU
;
Limin ZUO
;
Wei JIANG
;
Guixia LIU
;
Yang ZHANG
;
Yuan LI
;
Rong JIANG
- Publication Type:Journal Article
- Keywords:
Two-dimensional liquid chromatography;
Trichoderma hazianum;
Fermentation liquor;
2460 A
- From:
Chinese Journal of Analytical Chemistry
2014;(12):1828-1832
- CountryChina
- Language:Chinese
-
Abstract:
An on-line two dimensional liquid chromatographic (2D-LC) method was established by using an ultimate dual gradient liquid chromatography, three chromatographic columns and valve-switching technology to detect 2460A in trichoderma hazianum fermentation liquor. MF C8 column (10 mm×4. 6 mm, 5. 0 μm) was used as purification column and MG C18 column (20 mm×4. 6 mm, 5. 0 μm) was used as enriching column. Methanol and water were used as mobile phase with a gradient elution at a flow rate of 2. 0 mL/min. The sample was separated on the Thermo Hypersil GOLD C18 column (250 mm×4. 6 mm, 5. 0μm) maintained at 40 ℃ using methanol and water. The flow rate was 1. 0 mL/min and 1. 0 mL sample was injected into the 2D-LC system. The detection wavelength was 424 nm. The whole analytical time was less than 60 min. The standard curve was linear over the 2460A concentration range of 0. 0025-10. 0 mg/L(r=0. 9981, n=8). The limit of detection was calculated to be 1 . 2μg/L ( S/N=3 ) and the limit of quantification was calculated to be 2 . 5 μg/L ( S/N=10 ) . The average recoveries varied from 88 . 0% to 104 . 4%.
