Determination of Erythrocin in Chicken Manure by High Performance Liquid Chromatography-Corona-Charged Aerosol Detection Coupled with On-line Solid Phase Extraction
10.11895/j.issn.0253-3820.140734
- VernacularTitle:在线固相萃取结合高效液相色谱-电喷雾式检测器测定鸡粪中红霉素残留
- Author:
Qian ZHOU
;
Mantang CHEN
;
Lihua ZHU
;
Yaobin DING
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Corona-charged aerosol detector;
Online solid phase extraction;
Erythrocin;
Accelerated solvent extraction
- From:
Chinese Journal of Analytical Chemistry
2014;(12):1838-1841
- CountryChina
- Language:Chinese
-
Abstract:
A novel HPLC-CAD method coupled with on-line solid phase extraction ( SPE ) for the determination of erythrocin which was widely used in livestock farming was developed. After mixed with diatomite, 5. 0 g manure sample was put into the cell and extracted with hot water at 70℃ and 10. 3 MPa. An on-line SPE methodology was applied to pre-treat the sample, and the sample was seperated on an Acclaim 120 C18 column and analyzed by corona CAD detector using acetonitrile and 0. 1% formic acid as mobile phase. Good linearity for erythrocin was obtained in the range of 21-2000 μg/kg. The detection limit was 6. 3 μg/kg. The average recoveries were 79. 2%-87. 5%.
