Progress in Mass Spectrometry Acquisition Approach for Quantitative Proteomics
10.11895/j.issn.0253-3820.140821
- VernacularTitle:定量蛋白质组学质谱采集技术进展
- Author:
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Quantitative proteomics;
Synchronous precursor selection;
Parallel reaction monitoring;
Data independent acquisition;
Review
- From:
Chinese Journal of Analytical Chemistry
2014;(12):1859-1868
- CountryChina
- Language:Chinese
-
Abstract:
Mass spectrometry is an important and powerful tool for protein quantification. With the in-depth development of quantitative proteomics, limitations of classic MS based quantification methods, such as complicated matrix interference and throughput/capacity limitation, start to appear. Recent progress of series novel MS based techniques provide effective solutions for the limitations of relative and absolute proteomic quantification, including synchronous precursor selection ( SPS ) , mass defect isobaric labeling, parallel reaction monitoring ( PRM) , multiplexing acquisition ( MSX) , and various novel data independent acquisition ( DIA) modes. Here we summarized the current limitations of quantitative proteomics, reviewed the latest MS based quantification approaches, and discussed the features and advantages of these novel techniques for quantitative proteomic application.
