Dual-Gradient Liquid Chromatography-Tandem Charged Aerosol Detector for Detection of Macromolecules in Traditional Chinese Medicine Injections
10.11895/j.issn.0253-3820.140849
- VernacularTitle:双三元液相色谱-串联电喷雾检测器用于中药注射剂大分子物质筛查研究
- Author:
Tingting ZHANG
;
Yu WANG
;
Danhua GU
;
Li ZHANG
;
Yan JIN
- Publication Type:Journal Article
- Keywords:
Charged aerosol detection;
Liquid chromatography;
Size exclusion chromatography;
Macromolecule screening
- From:
Chinese Journal of Analytical Chemistry
2014;(12):1871-1872
- CountryChina
- Language:Chinese
-
Abstract:
A size exclusion chromatography method ( SEC) was established for the screening of macromolecules in traditional Chinese medicine (TCM) injections. In the experiment, TCM was collected through 0. 45μm hybrid filter and separated by SEC with MAbPac SEC-1 column. Acetonitrile was added by post-column mode with a flow rate of 0. 2 mL/min to enhance the detection sensitivity, and 25 mmol/L ammonium formate was used as the mobile phase at flow rate of 0. 2 mL/min. The charged aerosol detection was carried out with Dextran and Tween as the reference standards. The molecular weight distribution of Dextran ranged from 2500 to 133800 had a good linear relationship with the retention time. No macromolecules were detected out in the selected traditional Chinese medicine injections except Kuhuang injections.
