Application of Two-dimensional Liquid Chromatography Analysis of Drugs and Toxicants
10.11895/j.issn.0253-3820.140764
- VernacularTitle:二维液相色谱在药物和毒物分析中的应用进展
- Author:
Weibin XIAO
;
Yang JIAN
;
Hua LI
- Publication Type:Journal Article
- Keywords:
Two dimensional liquid chromatography;
Online solid phase extraction;
Pharmacokinetics;
Bioanalysis;
Review
- From:
Chinese Journal of Analytical Chemistry
2014;(12):1851-1858
- CountryChina
- Language:Chinese
-
Abstract:
Qualitative and quantitative analyses of biological samples containing drugs, toxicants and endogenous substances play an important role in the researches of life sciences, as well as in new drug discovery and development. Biological samples are characterized by complex matrix, multiple endogenous interferences, significantly lower concentrations of measured analytes compared to endogenous components and small sampling volume. Consequently, it often requires bioanalysis methods with superior specificity, high sensitivity and good reproducibility. The two-dimensional liquid chromatography (2D-LC) technique, which allows for high peak capacity, significant reduced matrix effect and carryover of complex matrix samples and automated sample pre-treatment and analysis, has been the powerful solution to the separation and analysis of biological sample and widely applied to environment, food and pharmaceutical analysis. On the basis of introduction of the principle and equipments of 2D-LC, the application of this technique in the pharmacokinetics, toxicological and biological study was reviewed.
