Comparison of transfection efficiency of two kinds of cells by lipofection and electroporation
10.3969/j.issn.1671-8348.2014.33.005
- VernacularTitle:脂质体法与电穿孔法转染两种细胞效率的比较
- Author:
Hexuan ZHANG
;
Keren SHAN
;
Yan HE
;
Ting ZHANG
;
Chanjuan WANG
;
Zhizhong GUAN
- Publication Type:Journal Article
- Keywords:
electroporation;
lipofection;
HepG2 cell;
SGC7901/ADM cell;
pcDNA3 .1 EGFP
- From:
Chongqing Medicine
2014;(33):4432-4433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the transfection efficiency between different transfection methods in human HepG2 and SGC7901/ADM cells so as to provide experimental basis for further study .Methods To electrons fect the enhanced GFP plasmid into HepG2 and SGC7901/ADM cells by lipofection and electroporation methods ,respectively .The survival rates and transfection efficiency were analyzed .Results The efficiency of eGFP vector transfected into HepG2 cells by lipofection was (23 .8 ± 2 .1)% , compared with lipofection method ,the efficiency of eGFP plasmid transfected by electroporation was up to (49 .6 ± 2 .5)% ,and the difference was statistically significant(P<0 .05) .The efficiency of SGC7901/ADM cells by lipofection was (25 .4 ± 1 .3)% ,com‐pared with lipofection method ,the efficiency of electroporation was up to(52 .6 ± 2 .1)% ,and the difference was statistically signifi‐cant(P<0 .05) .This study provides reliable test parameters for electransfection of HepG2 and SGC7901/ADM cells .Conclusion The transfection efficiency of large fragment vector is efficiently improved by electroporation .
