A Study on HPLC Fingerprints of Zingiber Officinale Before and After Sulfur-fumigation
10.3969/j.issn.1005-5304.2014.12.025
- VernacularTitle:生姜硫磺熏蒸前后HPLC特征色谱图研究
- Author:
Ping LI
;
Jun JIANG
;
Jian TANG
;
Huajun WANG
;
Liya GU
;
Haotian LI
;
Xiaobin JIA
- Publication Type:Journal Article
- Keywords:
zingiber officinale;
sulfur-fumigate;
6-gingerol;
fingerprint
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(12):85-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explain the differences between zingiber officinale and its sulfur fumigation products on chromatography fingerprints by HPLC-DAD;To discuss the influence of sulfur-fumigation on the quality of zingiber officinale. Methods HPLC, diode array detector, and ZORBAX SB-C18 column were used with acetonitrile-water as the mobile phase, gradient elute, volume flow rate of 1 mL/min, column temperature of 25 ℃, and detection wavelength of 280 nm. HPLC-DAD technology was applied to establish the fingerprints of zingiber officinale before and after sulfur-fumigating process, in order to analyze the HPLC fingerprints of zingiber officinale before and after sulfur-fumigating process. External standard method was used to do the quantitative determination of 6-gingerol. Results The 17 common peaks were identified through the comparison of 3 batches of fingerprints of zingiber officinale and their sulfur-fumigated samples. The peak areas of NO.3, NO.10, NO.11, and NO.17 were reduced by 50.68%, 64.41%, 67.68%, and 21.23%respectively. The content of 6-gingerol had no significant change. Conclusion The chemical composition of zingiber officinale changed at different degrees after sulfur-fumigated process. The safety and effectiveness of sulfur fumigation products of zingiber officinale require more researches.
