Effect of Hes1 on bone marrow CD34+cells in acute myeloid leukemia
10.3969/j.issn.1000-8179.20141339
- VernacularTitle:Hes1对急性髓系白血病患者骨髓CD34+CD38-细胞的作用及其机制研究
- Author:
Chen TIAN
;
Yongsheng JIA
;
Dongzhi HU
;
Yizhuo ZHANG
- Publication Type:Journal Article
- Keywords:
T-ALL;
CD34+CD38-cell;
Hes1;
cell cycle;
proliferation
- From:
Chinese Journal of Clinical Oncology
2014;(22):1422-1425
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the effect of Hes1 on bone marrow CD34+cells in acute myeloid leukemia (AML). Meth-ods:Bone marrow mononuclear cells were isolated by using Ficoll. Then, the proportion and cell cycle of CD34+cells were analyzed by using fluorescence-activated cell sorting (FACS). CD34+cells were cultured in vitro for colony-forming cells (CFC). The expression of Hes1 in CD34+cells was evaluated by using real-time polymerase chain reaction. After upregulating the expression of Hes1 in CD34+cells, the cell cycle was analyzed through FACS, and the colony formation of CD34+Hes1+cells was analyzed by CFC. Results:The ra-tio of CD34+cells in the bone marrow was lower in the AML group than in the control group. In addition, more CD34+cells underwent quiescence in the AML group than in the control group. In vitro assay showed that the colony formation of CD34+cells was lower in the AML group than in the control group. The expression of Hes1 was higher in the CD34+cells from the AML patients than that in the CD34+ cells from normal donors. After Hes1 transduction, more CD34+ cells underwent quiescence and showed weak proliferation. Conclusion:The proportion of CD34+cells in the bone marrow was lower in AML patients than in normal donors. A large proportion of CD34+cells underwent quiescence, which was related to Hes1, in AML patients.
