Proteomic analysis of nasal lavage fluid in patients with allergic rhinitis
10.3969/j.issn.1673-4130.2014.22.009
- VernacularTitle:过敏性鼻炎的鼻液蛋白质组学分析
- Author:
Fuyi XIE
;
Xiaoren ZHANG
;
Qi SUN
- Publication Type:Journal Article
- Keywords:
allergic rhinitis;
biomarkers;
proteomic
- From:
International Journal of Laboratory Medicine
2014;(22):3027-3029
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the biomarkers in nasal lavage fluid of patients with allergic rhinitis (AR) by proteomics tech‐nology .Methods The nasal lavage fluid samples of 10 cases of AR patients and 20 cases of normal controls were collected both be‐fore and after allergen stimulation tests .Two‐dimensioned gel eletrophoresis was used to detect the proteins in nasal lavage fluid samples .The proteins that showed different expression levels were identified by MALDI‐TOF/TOF mass spectrometry .Results Six kinds of proteins differentially expressed were screened out .The expressions of lactoferrin ,α1‐antitrypsin ,and APOA1 increased in the AR patients after being stimulated by allergens .On the contrary ,the expressions of PLUNC protein ,haptoglobin and IgJ de‐creased .Conclusion The six kinds of proteins might be the biomarkers of AR .
