Effects of Danzhi Jiangtang Capsule on CXCL9 and IL-8 in Diabetic Model Rats
10.3969/j.issn.1005-5304.2014.12.013
- VernacularTitle:丹蛭降糖胶囊对糖尿病模型大鼠趋化因子9和白细胞介素-8的影响
- Author:
Zhongnan LI
;
Yuanyuan XIONG
;
Li LI
;
Zhaohui FANG
- Publication Type:Journal Article
- Keywords:
diabetes mellitus;
Danzhi Jiangtang Capsule;
CXCL9;
IL-8;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(12):45-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Danzhi Jiangtang Capsule on CXCL9 and IL-8 in type 2 diabetic model rats, and discuss the mechanism in TCM intervention in type 2 diabetes. Methods Fifty male SD rats were randomly divided into 5 groups:normal control group, high-dosage group of Danzhi Jiangtang Capsule, low-dosage group of Danzhi Jiangtang Capsule, pioglitazone group and model group. Normal control group was fed with basal diet, while the other groups were fed with high fat diet. A single intraperitoneal injection of STZ of 35 mg/kg was employed to establish the animal model of diabetes mellitus. When the diabetic rodent model was built, high-dosage group of Danzhi Jiangtang Capsule and low-dosage group of Danzhi Jiangtang Capsule were treated with 1.08 g/(kg?d) and 0.54 g/(kg?d) by gavage, pioglitazone group was treated with 10 mg/(kg?d) by gavage, the blank group and the model group were treated with physiological saline 5 mL/(kg?d) by gavage for 8 weeks. CXCL9, IL-8 and blood glucose, and lipid in serum of rats were determined with double antibody sandwich method. Results Expressions of CXCL9 and IL-8 in model group increased significantly, compared with the normal control group (P<0.05, P<0.01). Expressions of CXCL9 and IL-8 in high and low dosage Danzhi Jiangtang Capsule group significantly decreased, the blood glucose and lipid also decreased, compared with model group (P<0.01). Conclusion Danzhi Jiangtang Capsule has inhibitory effects on the high expression of inflammatory factors in diabetes, and can also lower bloodglucose, and regulate blood lipid.
