Effects of Mieyou Soup on HSP70 and AQP4 in H.Pylori-Associated Gastritis Mice of Pi-Wei Hygropyrexia Syndrome
10.3969/j.issn.1005-5304.2014.12.016
- VernacularTitle:灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证小鼠热休克蛋白70和水通道蛋白4的影响
- Author:
Bin YU
;
Yan LUO
;
Xiaojuan WANG
;
Yin XU
;
Xuan GUO
;
Zhonghua DU
;
Rong XIA
;
Jiao YIN
- Publication Type:Journal Article
- Keywords:
Mieyou Soup;
HP associated gastritis;
Pi-Wei hygropyrexia syndrome;
HSP70;
AQP4;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(12):55-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressions of HSP70 and AQP4 in the H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome;To investigate the mechanism of Mieyou Soup. Methods The mice were randomly divided into high concentration of Mieyou Soup group, low concentration of Mieyou Soup group, gastric triad group, model group and control group. BALB/c mice of H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome model was established by composite factor. After modeling, the mice were administered for of continuous 14 days. The drug dosage of high concentration of Mieyou Soup group, low concentrations of Mieyou Soup group, and gastric triad group were 12.4, 6.2 g/kg, and 0.279 8 mg/kg, respectively. Western Blot was used to detect the expression of HSP70 protein, and immunohistochemistry was employed to detect the expression of AQP4 protein. Results Compared with control group, the expression of HSP70 and AQP4 in model group significantly increased (P<0.05);compared with the model group, the HSP70 in high concentration of Mieyou Soup group, low concentrations of Mieyou Soup group, gastric triad group increased, the AQP4 in high concentration of Mieyou Soup group, low concentrations of Mieyou Soup group, gastric triad group decreased, with statistical significance (P<0.01). Conclusion Mieyou Soup may play a role in treatment of H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome by raising the expression of HSP70, and reducing the expression of AQP4 protein expression.
