Effects of Bullatacin on Proliferation and Apoptosis of A549 Cell Line of Pulmonary Adenocarcinoma

VernacularTitle:番荔枝内酯对肺腺癌A549细胞周期及凋亡的影响
Author: Qian WAN ; Yinglu LIU ; Ming GE ; Yonghong ZHANG
Publication Type:Journal Article
Keywords: annonaceous acetogenins; Bullatacin; A549 cell; proliferation; apoptosis; MAPK signal pathway
From: Chinese Journal of Information on Traditional Chinese Medicine 2014;(12):59-62
CountryChina
Language:Chinese
Abstract: Objective To investigate the apoptosis induction of Bullatacin on A549 cell line of pulmonary adenocarcinoma. Methods The MTT assay was used to detect the growth inhibition rates of A549 cells cultured with Bullatacin in different concentrations (6.25, 12.5, 25, 50, 100μg/mL). 25μg/mL Bullatacin was used to culture A549 cells for 0, 12, 24, 48 h. The cell cycle distribution and apoptosis were measured by flow cytemetry. The protein expressions of ERK, JNK, and p38 were studied by Western blot. Results Dosage dependence was obviously showed after the different concentrations of Bullatacin were used to A549, and 25 μg/mL;Bullatacin blocked A549 cell in G0/G1 periods and induced its apoptosis. Compared with the blank group, protein expressions of P-ERK, P-JNK, and P-p38 were all increased by different degrees. Conclusion Bullatacin significantly inhibits the proliferation and induces the apoptosis of A549 cell. Its mechanism is related to activity of MAPK pathway thought the phosphorylation of the three protein kinases by Bullatacin.