Effect of Zhengan Xifeng Decoction on Raf-1 mRNA and Protein Expression in Spontaneously Hypertensive Rats

VernacularTitle:镇肝熄风汤对自发性高血压大鼠Raf-1 mRNA和蛋白表达的影响
Author: Xin XIE ; Lin ZHANG ; Shiyu CHEN ; Fang YANG ; Lide ZHANG
Publication Type:Journal Article
Keywords: Zhengan xifeng decoction; Hypertension,spontaneous; Raf-1 mRNA
From: Herald of Medicine 2014;(11):1423-1426
CountryChina
Language:Chinese
Abstract: Objective To study the effect of different doses of Zhengan xifeng decoction on Raf-1 mRNA and protein expression in the cardiovascular tissue of spontaneously hypertensive rats( SHR). Methods A total of 50 male SHR,24 weeks old,were randomly divided into the model,low dose,medium dose,high dose of Zhengan xifeng decoction and the compound apocynum groups,10 in each group. Ten homologous male rats( WKY)served as the normal control group. After gavaged for 5 weeks,western blotting and RT-PCR were used to detect the Raf-1 protein and mRNA expression in the cardiovascular tissue,respectively. Results Compared with the model control group,both Raf-1 protein and mRNA expressions significantly increased in all treatment groups( P〈0. 01 ). Conclusion The Zhengan xifeng decoction can stimulate cell proliferation and inhibit cell apoptosis by up-regulating the expression of Raf-1.