Effects of Resveratrol Combined with Radiotherapy on Human Hepatoma Cell Line Bel-7404 in Vitro

VernacularTitle:白藜芦醇对肝癌Bel-7404细胞体外放射治疗的影响
Author: Renbing BI ; Deliang GUO ; Dingyu PAN ; Zhisu LIU ; Shouguo HU
Publication Type:Journal Article
Keywords: Resveratrol; Apoptosis; Hepatoma cells Bel-7404; Radiotherapy sensitization
From: Herald of Medicine 2014;(11):1442-1445
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of radiotherapy combined with resveratrol( Res)on proliferation, invasion and apoptosis of hepatoma cell line Bel-7404. Methods Bel-7404 cell line was treated with Res(25 μmol·L-1 ) combined with radiotherapy,then divided into four groups( group 1:the control;group 2:radiation at dose of 2 Gy;group 3:radiation at dose of 4 Gy;group 4:radiation at dose of 6 Gy). Cell proliferation and invasion were detected by MTT assay. Cell apoptosis were observed by fluorescence microscopy. Expressions of MMP-2 and VEGF proteins were determined by Western blotting. Results Compared with the control group,cell proliferation and invasion were significantly inhibited,while cell apoptosis was increased in all radiation groups(P〈0. 05). Conclusion The sensitivity of hepatoma Bel-7404 cells to radiation can be enhanced by resveratrol. Radiation therapy combined with resveratrol can inhibit proliferation and invasion of hepatoma cells and increase the cell apoptosis,which may be related with the down-regulation of MMP-2 and VEGF proteins.