Expression and clinical significance of serum microRNA-100 in gastric cancer patients
10.3969/j.issn.1000-8179.20140777
- VernacularTitle:MicroRNA-100在胃癌患者血清中的表达及临床意义
- Author:
Liping WANG
;
Qizhi WANG
;
Min DENG
;
Shanjun YAN
;
Jianchao WANG
;
Dapeng LI
;
Qiangwu WANG
- Publication Type:Journal Article
- Keywords:
MicroRNA;
miR-100;
tumor marker;
stomach neoplasm;
sensitivity and specificity
- From:
Chinese Journal of Clinical Oncology
2014;(24):1587-1590
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of microRNA-100 (miR-100) in the serum of gastric cancer patients and its clinical significance. Methods: At The First Affiliated Hospital of Bengbu Medical College, 40 gastric cancer patients who underwent surgery with complete follow-up data and 40 healthy controls were selected as research subjects. Total miRNA was isolated from the se-rum samples of the cancer patients. A stable and sensitive detection method for the absolute quantification of miR-100 was established. The serum levels of miR-100 in the patients and healthy controls were tested according to this novel method. Differences of the miR-100 expression in the serum samples of the patients and healthy controls were analyzed using statistical analysis. The correlation between miR-100 expression levels and the clinicopathological features of gastric cancer was also analyzed. Results: The expression level of miR-100 in the serum was significantly higher in cancer patients[(2.78±1.92) fmol/L]than in the healthy controls[(0.19± 0.15) fmol/L, P<0.01]. The receiver operating characteristic curve of miR-100 indicated that serum miR-100 has satisfactory specificity and sensitivity for the diagnosis of gastric cancer (AUC=0.985). Further analytical results indicated that no significant differences were found among the expression levels of serum miR-100 with respect to the gender, age, tumor diameter, infiltrating depth, differentiation degree, frequency of lymph-node metastasis, and TNM stage (P>0.05). Conclusion: Serum miR-100 testing may be helpful in the diag-nosis of gastric cancer.
