Transcription level of mitochondria gene Cyt b in different stages of Pagumogonimus skr j abini
10.3969/cjz.j.issn.1002-2694.2014.12.003
- VernacularTitle:斯氏狸殖吸虫线粒体Cytb基因在不同虫期的转录水平分析
- Author:
Man WANG
;
Xilin ZHANG
;
Yuqing HUANG
;
Wenbi CHEN
;
Junping SHE
;
Li XIANG
;
Guangxi WANG
- Publication Type:Journal Article
- Keywords:
Pagumogonimus skrjabini;
mitochondria Cytb gene;
real-time PCR;
transcriptional level
- From:
Chinese Journal of Zoonoses
2014;(12):1187-1191
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:In this study ,we aimed to analyze the transcription level of mitochondria gene Cytb in metacercariae ,larva‐30d ,larva‐60d ,adult ,and egg of Pagumogonimus skrjabini .The mRNA of metacercariae ,larva‐30d ,larva‐60d ,adult and egg of P .skrjabini were extracted with genomic extraction kit ,and transcripted reversely into cDNA .With 18SrDNA of Par‐agonimus westermani as an internal standard primer ,Cytb genes were amplified by real‐time PCR for establishing standard curves to evaluate the copy number of genes .Those quantitative analyses were reliable because the R value of standard curves was 0 .994 (>0 .98) ,and the melting curve showed a single peak .There were increasing trend of transcription of Cytb gene at metacercariae stage ,larva‐30d stage ,and larva‐60d stage .There were less transcription of Cytb gene at adult stage and no transcription at egg stage .There were differences about the transcriptional level of mitochondria Cytb gene at different stages of Pagumogonimus skrjabini ,and peaked at larva‐60d stage ,suggesting that Cytb gene may play a role in the development and migrating of larva .
