Nested-PCR and LAMP in detection of B .burgdor f eri in ticks
10.3969/cjz.j.issn.1002-2694.2014.12.04
- VernacularTitle:巢式PCR与LAMP方法在蜱的莱姆病螺旋体检测中的应用
- Author:
Lin ZHANG
;
Xuexia HOU
;
Zhen GENG
;
Qin HAO
- Publication Type:Journal Article
- Keywords:
ixodes;
Borreliaburgdorferi;
nested PCR;
LAMP;
positive rate
- From:
Chinese Journal of Zoonoses
2014;(12):1192-1195
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Nested‐PCR and loop‐mediated isothermal amplification (LAMP) were applied to identify the Borrelia burg‐dor f eri (B .burgdor f eri) in ticks in this study .A total of 112 adult ixodes were collected from vegetation in a forest area and farm animals in Xunhua County ,Qinghai Province and Xinbin County ,Liaoning Province .The ticks were examined for the presence of B .burgdorferi by nested‐PCR and LAMP .Results showed that 12 in 51 samples were found positive in Xunhua County (23 .53% ) .While positive rate in Xinbin County was 29 .51% with 18 samples positive in 61 samples .In total of 112 tick samples ,the PCR‐positive rate was 17 .86% with 20 positive samples identified ,whereas 15 positive samples were con‐firmed with positive rate of 13 .39 % by LAMP assay .There was no significant difference between the two assays (Х2 =0 .85 , P>0 .05) .Results suggest that both nested‐PCR and LAMP could be used in identifying B .burgdorferi in ticks .Combina‐tion of these two assays could improve the testing results .This is the first report of B .burgdorferi in ticks in Xunhua and Xinbin counties ,and helps to complete the database of the infection rate of B .burgdor f eri in ticks in the widely‐forested area of China .
