Role of NF-κB in diabetic neuropathy
10.3969/j.issn.1000-4718.2014.10.010
- VernacularTitle:NF-κB 在糖尿病性神经痛中的作用及机制
- Author:
Yangliang HUANG
;
Yi ZHONG
;
Jingxin HU
- Publication Type:Journal Article
- Keywords:
Diabetic neuropathy;
NF-kappa B;
Voltage-gated sodium channels;
Dorsal root ganglion
- From:
Chinese Journal of Pathophysiology
2014;(10):1784-1788
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of NF-κB in diabetic neuropathy.METHODS: The diabetic rats were induced by intraperitoneal injection of streptozocin ( STZ) .The pain behavior test was used to detect the mechanical and thermal withdraw threshold of the rats’ bilateral hind paws.The protein levels of p-NF-κB and t-NF-κB in the rats’ L4 and L5 dorsal root ganglions ( DRG) were determined by Western blotting.The expression of Nav1.7 in DRG of diabetic neuropathy rats with or without NF-κB inhibitor PDTC was detected by the method of immunohistochemistry.RESULTS:The mechanical and thermal withdraw threshold of bilateral hind paws in the diabetic rats was decreased from 4 weeks to 12 weeks after injection of STZ.The protein levels of p-NF-κB in L4 and L5 DRG were significantly increased in the rats with diabetic neuropathy.Intrathecal administration of NF-κB inhibitor PDTC attenuated the increase in p-NF-κB and Nav1.7 in L4 and L5 DRG.Pain behaviors were also alleviated by PDTC.CONCLUSION:The increase in p-NF-κB is closely rela-ted to the generation of diabetic neuropathy.Inhibition of NF-κB blocks pain behaviors and the over-expression of Nav1.7 in DRG.
