Seipin gene deficiency induces renal injury in mice

VernacularTitle:Seipin 基因缺陷诱发小鼠肾损害
Author: Xiaoyue WU ; Huan WANG ; Xuejing LIU ; Jiawei LIAO ; Ling ZHANG ; Guoqing LIU ; Wei HUANG
Publication Type:Journal Article
Keywords: Seipin; Lipodystrophy; Renal injury
From: Chinese Journal of Pathophysiology 2014;(10):1850-1854
CountryChina
Language:Chinese
Abstract: AIM:To investigate the effects of seipin gene deficiency on renal injury and the possible mecha-nisms in seipin-/-mice.METHODS:Six-month-old male seipin knockout ( seipin-/-) and wild-type ( WT) mice ( n=8) were used to study 24 h urinary albumin excretion ( UAE) , renal functions, pathological changes, and plasma leptin and adiponectin levels.Seipin mRNA expression in different tissues and each part of the kidney was also measured in WT mice.RESULTS:Real-time PCR analysis showed seipin mRNA expression in WT mice was higher in adipose tissue and testicles, and was also found in the kidney, which was mainly in glomeruli.Compared with control group, seipin-/-group showed increased kidney weight/tibia length (P<0.01), 24 h UAE (P<0.01), creatinine clearance (P<0.01), and glomerular and mesangial surface area (P<0.05).Both plasma leptin (P<0.01) and adiponectin (P<0.05) levels were significantly decreased in seipin-/-mice.CONCLUSION:Seipin gene deficiency in mice leads to renal injury prob-ably by decreasing plasma leptin and adiponectin levels due to lack of adipose tissue.