Inmbition of nodosin on cell proliferation and expression of Bcl-2 and Bax in HepG2 cells
10.3969/j.issn.1000-4718.2014.10.026
- VernacularTitle:Nodosin对HepG2细胞株的增殖抑制作用及对Bcl-2和Bax表达的影响
- Author:
Guangfan HAI
;
Bingxuan NIU
;
Pinpin LI
;
Lanqing GUO
;
Taizhen CUI
;
Shengying LI
- Publication Type:Journal Article
- Keywords:
HepG2 cells;
Nodosin;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2014;(10):1879-1882
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of nodosin extracted from Chinese traditional medicine on the pro-liferation of HepG2 cells cultured in vitro and to detect the protein expression of Bcl-2 and Bax in HepG2 cells.METH-ODS:HepG2 cells were treated with different concentrations (1.25, 2.5, 5, 10 and 20 μmol/L) of nodosin for 24 h. The morphological changes of HepG2 cells were observed under inverted microscope.The inhibitory rates of HepG2 cell growth were detected by MTT assay.The apoptotic rates and the protein expression of Bcl-2 and Bax were analyzed by flow cytometry.RESULTS:Shrunken and suspended HepG2 cells increased with the increases in the concentrations of nodo-sin.The apoptotic rates and the expression of Bax increased with the increases in the doses of nodosin, while the expression of Bcl-2 decreased.CONCLUSION:Nodosin inhibits the growth of HepG2 cells in a dose-dependent manner.The inhibi-tion of HepG2 cell growth is induced by decreasing Bcl-2 and increasing Bax, thus promoting cell apoptosis.
