Lentivirus-based RNAi and its application in the study of T lymphocytes
10.3969/j.issn.1000-4718.2014.10.032
- VernacularTitle:慢病毒载体介导的RNA干扰及其在T淋巴细胞的应用
- Author:
Jingjing DU
;
Yihua SUN
- Publication Type:Journal Article
- Keywords:
Lentivirus;
RNA interference;
T lymphocytes
- From:
Chinese Journal of Pathophysiology
2014;(10):1905-1909
- CountryChina
- Language:Chinese
-
Abstract:
The technique of lentivirus-based RNAi ( RNA interference) is a combination of the effective infec-tion and integrating characteristics of lentivirus vector and the specificity of RNAi to silence a homologous gene expression. It caused the post-transcriptional gene silencing ( PTGS) by a specific target sequence of mRNA degradation.As a kind of commonly used target cells in gene therapy, T lymphocytes play an important role in graft-versus-host disease ( GVHD) and other diseases.It is of great significance for increasing the infection efficiency of the target gene to T lymphocytes on this kind of disease for gene therapy.In this paper, we summarize the application of lentivirus-based RNAi in T lymphocytes.
