The Expression of HDAC2 In PeripheraI BIood MononucIear CeIIs from Patients with Sudden SensorineuraI Hearing Loss
10.3969/j.issn.1006-7299.2014.06.001
- VernacularTitle:难治性突发性聋患者外周血单个核细胞内HDAC2表达与其预后的相关性分析
- Author:
Jie HOU
;
Yanhong DAI
;
Lisheng XIE
;
Qiongqiong ZHOU
;
Wandong SHE
- Publication Type:Journal Article
- Keywords:
SSNHL;
Glucocorticoid resistance;
PBMC;
HDAC2;
Post-translational modification
- From:
Journal of Audiology and Speech Pathology
2014;(6):559-563
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate expression of HDAC2 in peripheral blood mononuclear cells(PBMCs)from glucocorticoid-resistant versus glucocorticoid-sensitive patients with sudden sensorineural hearing loss and identi-ty the relationship between the level of HDAC2 and glucocorticoid insensitivity.Methods PBMCs were collected from10 patients with deviation of nasal septum (control group)and 20 sudden sensorineural hearing loss patients be-fore and after intratympanic methylprednisolone for 10 days.We divided the SSNHL patients into 2 groups (GC sensitive group and GC insensitive group)according to their hearing recovery.Real time PCR and HDAC2 Assay Kit were used to detect the expression level of HDAC2 mRNA and HDAC2 activity in PBMCs.The data were analyzed with SPSS 17.0 software.ResuIts Before intratympanic methylprednisolone,the level of HDAC2 activity were sig-nificantly depressed in SSNHL patients,while the HDAC2 mRNA expressing much higher than the control group. The expression level of HDAC2 mRNA increased significantly after intratympanic methylprednisolone.The HDAC2 activity in GC sensitive group increased significantly.ConcIusion Knockdown of HDAC2 expression induces corti-costeroid in-sensitivity.Glucocorticoids can increase the expression of HDAC2 mRNA.HDAC2 activity can be down-regulated by post-translational modifications.
