A Study on Fingerprint of Citri Reticulatae Decoction Pieces by HPLC
10.3969/j.issn.1005-5304.2014.12.024
- VernacularTitle:青皮饮片HPLC指纹图谱研究
- Author:
Jin LI
;
Xiaoli ZHAO
- Publication Type:Journal Article
- Keywords:
Citri Reticulatae;
decoction pieces;
HPLC;
fingerprint
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(12):82-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an analytical method for fingerprint of Citri Reticulatae decoction pieces by HPLC. Methods Thermo C18 chromatographic column (4.6 mm×250 mm, 5μm) was used with methanol-0.3%phosphoric acid in gradient elution. The flow rate was 0.8 mL/min, the detection wavelength was set at 280 nm, and the temperature was 40 ℃. The similarities of the 15 batches of samples were compared by similarity evaluation, cluster analysis and principal component analysis. Results Based on the fingerprints of Citri Reticulatae decoction pieces, 8 common peaks were identified. The similarities were all more than 0.9 among all batches. 15 batches can be divided into three categories. Conclusion The method is simple and steady. It provides a basis for the quality control and evaluation of Citri Reticulatae decoction pieces.
