Analgesia and Anti-inflammatory Effect of Total Flavonoids Extracted from Euphorbia prolifera
10.3870/yydb.2014.11.003
- VernacularTitle:松生等总黄酮提取工艺及镇痛抗炎作用研究
- Author:
Yan JIN
;
Shan HUANG
;
Lei ZHAO
;
Xiaona PEI
- Publication Type:Journal Article
- Keywords:
Euphorbia prolifera;
Total flavonoids;
Reflux extraction;
Analgesia;
Anti-inflammation
- From:
Herald of Medicine
2014;(11):1411-1415
- CountryChina
- Language:Chinese
-
Abstract:
Objective The study aimed to investigate the best extraction process and the analgesia and anti-inflammatory properties of total flavonoids from Euphorbia prolifera. Methods Through single factor experiment and orthogonal test,factors that affect the extraction yield of total flavonoids were studied,including the extraction time,solid to liquid ratio, ethanol concentration and extraction times. The mice models of ear edema induced by xylene and twisting induced by acetic acid were used to evaluate the analgesia and anti-inflammatory effects of different extractions from Euphorbia prolifera. Results Total flavonoids were extracted by the refluxing method,and the optimum conditions were extracting for 3 hours,solid to liquid ratio of 1:30,ethanol content of 60% in the solvent,and processed for 2 times. The highest extraction yield of total flavonoids from Euphorbia prolifera was 5. 63%. The ethanol,ethyl acetate and n-butanol extracts decreased twisting and ear swelling in mice. Conclusion The extraction for total flavonoids from Euphorbia prolifera is simple,efficient and reproducible. The ethanol,ethyl acetate and n-butanol extracts of total flavonoids have obvious analgesia and anti-inflammatory effects.
