Effects of Morphine on μOpioid Receptor-mediated ERK Phosphorylation

VernacularTitle:吗啡对μ阿片受体介导的ERK磷酸化的影响
Author: Jiaming BIAN ; Mei ZHANG
Publication Type:Journal Article
Keywords: Morphine; μ opioid receptor; Ovary cells; ERK; Phosphorylation
From: Herald of Medicine 2014;(11):1446-1449
CountryChina
Language:Chinese
Abstract: Objective To study the phosphorylation mode of extracellular regulated kinase( ERK)induced by acute and chronic morphine treatment on Chinese hamster ovary( CHO)cells expressed withμopioid receptors. Methods The time course of ERK phosphorylation 1 h and 36 h after morphine exposure as well as naloxone-precipitated withdrawal was detected by immunobloting. Results A transient enhancement of ERK phosphorylation was induced by 1 μmol · L-1 morphine with the peak effect at 5 min(P〈0. 01),and the effect was dose-dependent. No difference in ERK phosphorylation was found after 36h of treatment with 10 μmol · L-1 morphine compared with the control. However,5 or 10 min-naloxone precipitation induced remarkable decrease in ERK phosphorylation compared with the control(P〈0. 01). Conclusion Different changes of ERK phosphorylation were found under acute and chronic morphine treatment and naloxone precipitation,indicating a compensation of ERK related pathway induced byμ opioid receptors.