Role of miR-184 in oxygen-glucose-deprivation induced SK-N-SH cell ischemic injury and its regulation on AKT2 level

VernacularTitle:miR-184在氧糖剥夺诱导SK-N-SH细胞缺血损伤中的作用及其对抗凋亡基因AKT2表达的调节
Author: Xiaomin PANG ; Huiyao XIANG ; Jingli LIU
Publication Type:Journal Article
Keywords: Oxygen-glucose-deprivation; miR-184; AKT2; Ischemic injury
From: The Journal of Practical Medicine 2014;(20):3219-3222
CountryChina
Language:Chinese
Abstract: Objective To explore the role of miR-184 in Oxygen-Glucose-Deprivation (OGD) induced SK-N-SH cell ischemic injury and its regulation on AKT2 level. Method We used a combination of oxygen and glucose deprivation to imitate ischemic conditions in vivo. MiR-184 mimic and inhibitor were transfected into SK-N-SH cell to alter miR-184 levels. The expression of miR-184 and AKT2 were determined by using Real-time PCR. The extent of SK-N-SH cell survival rate was assessed by thiazolyl blue tetrazolium bromide (MTT) assay. Result Here, we observed that miR-184 was significantly inhibited in SK-N-SH cell after OGD (P<0.05). The changes of miR-184 level altered the expression of AKT2 mRNA. In addition, alteration of miR-184expressionsignificantly affected cell survival rate after OGD. Conclusion miR-184 plays an important role in ischemic injury through negatively regulating AKT2 level, which may provide a potential therapeutic target for ischemic stroke in miRNA levels.