High-mobility group box protein1 increases tumor necrosis factorαexpression in human bronchial epithelial cells
10.3969/j.issn.1006-5725.2014.20.004
- VernacularTitle:高迁移率族蛋白B1结合高级糖基化终产物受体可增加人支气管上皮细胞肿瘤坏死因子α的表达
- Author:
Yue LIANG
;
Changchun HOU
;
Hong HUANG
;
Lihong WU
;
Yiqiang CHEN
- Publication Type:Journal Article
- Keywords:
High mobility group box 1 (HMGB1);
human bronchial epithelial cells;
tumor necrosis factorα(TNF-α);
receptor for advanced glycation end products(RAGE)
- From:
The Journal of Practical Medicine
2014;(20):3213-3215
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of high-mobility group box protein1 (HMGB1) on the expression of TNF-αand its mechanism in 16HBE in vitro. Methods groups with different HMGB1 (0, 100, 500, 2 000 ng/mL) concentration was set; RAGE antagonizing groups were as control, HMGB1-2000ng, anti-RAGE and anti-RAGE+HMGB1. The changes of TNF-αmRNA and secretion were determined by quantitative PCR and ELISA. RAGE protein level was measured by western blotting. Results HMGB1 intervention and TNF-α expression of 16HBE presented a positive dose-dependent relationship. Thechanges of RAGE was HMGB1positively concentration dependent. In comparison with HMGB1 2 000 ng/mL group, anti-RAGE+HMGB1showed a remarkable reduction of TNF-α secretion. Conclusion In vitro, HMGB1 increases TNF-α expression in 16HBE with a dose-dependent manner through RAGE.
