Effect of temozolomide on the glioma invasiveness and its possible mechanism
10.13315/j.cnki.cjcep.2014.11.014
- VernacularTitle:替莫唑胺对胶质瘤侵袭性的影响及可能机制
- Author:
Jian WANG
;
Yongsen JIA
;
Xiqing ZHAO
;
Lijuan QIN
- Publication Type:Journal Article
- Keywords:
glioma;
tumor necrosis factor-α;
temozolomide;
invasiveness;
apoptosis;
p53
- From:
Chinese Journal of Clinical and Experimental Pathology
2014;(11):1259-1262
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the role of tumor necrosis factor-α( TNF-α) in the process of temozolomide ( TMZ) reduce glioma in-vasiveness and its possible mechanism. Methods C6 glioma cells of logarithmic phase were randomly divided into TMZ treatment (10, 30, 60, 120, 180, 240 min group) (n=15), dynamic monitoring content of TNF-αin the culture medium was measured by ra-dioimmunoassay, expression of p53 protein in C6 cells was detected with Western blotting method, and cell apoptosis was used with AnnexinV-FITC. A glioma invasiveness model was established in vitro and glioma invasiveness was determined by crystal violet stai-ning. Results For C6 cells, contents of TNF-αin the nutrient fluid and expressions of p53 protein in C6 cells obviously increased af-ter TMZ treatment and they achieved the peak at 120 min (P<0. 01), followed by decrease gradually. Glioma invasiveness was re-duced after TMZ acted on glioma in vitro. Conclusion TMZ can reduce glioma invasiveness by TNF-α, which this role may be is TMZ promote C6 cells release of TNF-α and increased TNF-α due to glioma cells apoptosis.
