Inhibitory effect of 15-oxospiramilactone on renal cell carcinoma 786-0 cells in vitro
- VernacularTitle:15-氧代绣线菊内酯对肾癌786-0细胞增殖的抑制作用
- Author:
Xiaoming YI
;
Song XU
;
Haowei HE
;
Longxin WANG
;
Chaopeng TANG
;
Zhengyu ZHANG
;
Zhengyu XU
;
Jingping GE
;
Wenquan ZHOU
- Publication Type:Journal Article
- Keywords:
Renal cell carcinoma;
Wnt signaling;
15-oxospiramilactone;
Apoptosis
- From:
Journal of Medical Postgraduates
2014;(12):1250-1253
- CountryChina
- Language:Chinese
-
Abstract:
Objective Wnt signaling plays an important role in the development and progression of renal cell carcinoma (RCC).This study aimed to evaluate the effects of the Wnt signaling inhibitor 15-oxospiramilactone on the proliferation , migration, cell apoptosis, and cycles of the human RCC cell line 786-0, and to investigate the possible mechanisms of this small molecule acting on RCC in ivtro. Methods We treated 786-0 cells with DMSO ( blank control group ) and 15-oxospiramilactone at the concentrations of1.25μmol/L (low 15 -OSL), 2.5μmol/L (medium 15-OSL), and 5μmol/L (high 15-OSL), respectively, for 72 hours.Then we observed the changes in the proliferation and migration of the 786-0 cells by MTT and scratch-wound assay and determined their apopto-sis and cycles by Annexin V-FITC/PI assay and flow cytometry . Results 15-oxospiramilactone significantly inhibited the growth of the 7860-cells, with the IC 50of 1.088 μmol/L at 72 hours, and decreased their migration distance (P<0.05).After 36 hours of treatment, the apoptosis rates of the 786-0 cells in the low, medium, and high 15-OSL groups were (12.17 ±0.56), (18.54 ± 1.07), and (50.74 ±1.28) %, respectively, significantly increased as compared with (7.85 ±0.42) %in the blank control group (P<0.05), and in an obviously concentration-dependent manner.15-oxospiramilactone remarkably reduced the number of cells in the G0/G1 phase and increased that in the G 2/M phase (P<0.05). Conclusion 15-oxospiramilactone can significantly inhibit the pro -liferation and migration and induce the apoptosis of 786-0cells in vitro.It may be a potential anti-RCC agent.
