Detection of differentially expressed microRNA profile in serum of patients with schizophrenia
- VernacularTitle:精神分裂症患者血清差异 microRNA 谱的检测
- Author:
Ying PENG
;
Yuanshuai HUANG
;
Zhenghua DENG
;
Song CHENG
;
Xianyong WEN
;
Kaizheng WANG
- Publication Type:Journal Article
- Keywords:
Schizophrenia;
microRNA;
Serum
- From:
Journal of Medical Postgraduates
2014;(12):1290-1293
- CountryChina
- Language:Chinese
-
Abstract:
Objective No uniform standards has been established for the clinical diagnosis of schizophrenia .The article was to investigate the differentially expressed microRNA ( miRNA) profile and explore its clinical significance . Methods Differentially expressed miRNAs were screened by FlashTag TM Biotin RNA chips and SAM software in serum of patients with schizophrenia and healthy controls , then the validation was performed by real-time fluorescent quantitative reverse transcription polymerase chain reaction ( RT-PCR) . Results Three differentially expressed miRNAs were screened , including up-regulated hsa-miR-1281 ( fold change =1.50881) and two down-regulated miRNAs:hsa-miR-2861(fold change=0.642) and hsa-miR-638 (fold change=0.516).The com-parative analysis of RT-PCR by SPSS 17.0 Kruskal Wallis H Test validated the expression levels of hsa-miR-2861 and hsa-miR-638 in patients with schizophrenia decreased significantly in comparison to healthy controls (χ2 =4.089,χ2 =4.083, P<0.001).While the expression level of hsa-miR-1281 in patients with schizophrenia was in higher expression level compared with control group (χ2 =5.333, P<0.001). Conclusion There are differentially expressed miRNA profile in serum of patients with schizophrenia , in which miR-1281 , miR-2861 and miR-638 could be new serum markers for diagnosis of schizophrenia .
