The role of endoplasmic reticulum stress in the hyperoxia-induced lung injury in premature rats
10.3969/j.issn.1000-3606.2014.12.019
- VernacularTitle:内质网应激与高氧诱导早产大鼠肺损伤的作用
- Author:
Qin WANG
;
Wenbin DONG
;
Zhongli CHE
;
Na HE
;
Li YU
;
Qingping LI
;
Xuesong ZHAI
;
Xiaoping LEI
- Publication Type:Journal Article
- Keywords:
hyperoxia-induced lung injury;
endoplasmic reticulum stress;
cell apoptosis;
premature rat
- From:
Journal of Clinical Pediatrics
2014;(12):1171-1175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of endoplasmic reticulum (ER) stress in the hyperoxia-induced lung injury in premature rats. Methods Forty-eight premature Wistar rats were randomized into two groups 12 hours after birth:hyperoxia group (n=24) inhaled 95%oxygen and control group (n=24) inhaled air. Eight rats were sacriifced in each group on day 1, 3, 7 after the treatment and the left lungs were embedded. The pathological changes in the HE stained sections of lung tissues were observed. The expressions of ER related protein ERp57 and c/EBP homologous protein CHOP were detected by immuno histo-chemistry and the apoptosis of lung cells was detected by TUNEL analysis. Results The typical pathological characteristics of acute lung injury were observed in hyperoxia group. The expressions of ERp57 and CHOP were increased with the exposure time in hyperoxia group, and were signiifcantly higher than in control group (P<0.05). The apoptosis rate of lung cells in hyperoxia group was signiifcantly higher than in control group (P<0.01). There was signiifcant positive correlation between cell apoptosis index and expressions of Erp57 and c/EBP homogeneous protein. Conclusions ER stress initiated apoptosis participates and plays an important role in the process of hyperoxia-induced lung injury in premature rats.
