Polylactic-co-glycolic acid complex with different concentrations of Schwann cells for peripheral nerve regeneration
10.3969/j.issn.2095-4344.2014.47.007
- VernacularTitle:不同浓度许旺细胞与聚乳酸-聚羟基乙酸共聚物修复损伤周围神经的比较
- Author:
Huanwei SUN
;
Tiehui ZHANG
;
Xinyan YOU
;
Yuanfei REN
;
Sheng ZHONG
- Publication Type:Journal Article
- Keywords:
peripheral nerves;
Schwann cels;
sciatic nerve
- From:
Chinese Journal of Tissue Engineering Research
2014;(47):7579-7584
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have shown that nerve grafts with Schwann cels can repair peripheral nerve defect and Schwann cels have an important role in nerve regeneration. OBJECTIVE: To observe the rehabilitation status of neutral function after sciatic nerve injury in rats bridged by nerve grafts with Schwann cels. METHODS: A rat model of sciatic nerve injury was established, and schwann cels were primarily cultured. Then, the rat model was repaired with polylactic-co-glycolic acid copolymer-extracelular matrix gel-Schwann cels complex. According to different concentrations of Schwann cels, there were five cel groups from 105/L to 109 RESULTS AND CONCLUSION: The nerve conduction velocities in the cel groups were al higher than that in the control group at 3, 6, 12 weeks after modeling (P < 0.01), and it was highest in the 10 and a control group. The nerve conduction velocity was detected respectively at 3, 6, 12 weeks after modeling; the e tibialis anterior muscle gravity was detected and histological observation was done at 12 weeks. 8/L (P < 0.05). At 12 weeks, hematoxylin-eosin staining of the tibialis anterior muscle showed that the number of normal muscle fibers was higher in the cel groups than the control group (P < 0.05). In the 108/L and 109 and had similar length, thickness and density. These findings indicate that polylactic-co-glycolic acid complex with 10/L groups, the morphology of tibialis anterior muscle recovered wel; the muscle fibers were in strip-like and wavy shapes, grew in the same direction, 8/L Schwann cels is better to promote sciatic nerve regeneration.
