DNA barcoding of rodent in Ningxia Hui Autonomous Region,China

VernacularTitle:宁夏啮齿动物DNA条形码分析
Author: Tao ZHANG ; Wenyan NING ; Jianhua WU ; Jingli LIU ; Fugui QIAO ; Guichang LI ; Liang LU ; Qiyong LIU ; Li LI ; Jianhua ZHAO
Publication Type:Journal Article
Keywords: DNA barcoding; nature focus; rodent; implied species; Ningxia
From: Chinese Journal of Zoonoses 2014;(12):1234-1238
CountryChina
Language:Chinese
Abstract: ABSTRACT:To improve the ability of correct classification and identification of rodents in different kinds of foci of zoonosis in Ningxia Hui Autonomous Region in China ,rodents samples from different habitats of Ningxia were collected .Sequences of COI gene were amplified and sequenced from 154 samples .Based on these sequences ,the pairwise genetic distance were calcu‐lated ,and a Neighbor Joining tree were constructed .According to the NJ tree ,20 clusters with high bootstrap support were found from 19 morphological species .The striped dwarf hamsters were divided into two clusters ,which suggested that there were two cryptic species with stripe on the back .The pika from Helan Mountain showed close relationship with Ochotona pal‐lasi ,and the genetic distance was as low as 3 .6% .Results show that DNA barcodes could be used to accurately identify speci‐mens of rodents and correct morphological identification errors .It could discover appearance indistinguishable implied species and could better study the classification and evolution of rodents .