Prediction and identification of B-cell epitopes on monkey B virus envelope protein gD
10.3969/j.issn.1671.7856.2014.011.005
- VernacularTitle:猴B病毒囊膜蛋白gD的B细胞表位预测及鉴定
- Author:
Huahu YE
;
Jufang YUAN
;
Huan LIU
;
Yujun FAN
- Publication Type:Journal Article
- Keywords:
Epitope prediction;
Enzyme-linked immunosorbent assay;
Monkey B virus;
Envelope protein gD
- From:
Chinese Journal of Comparative Medicine
2014;(11):20-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Identify B-cell epitopes on monkey B virus envelope protein gD.Methods Base on bioinformatics software, secondary structure, hydrophilicity, surface accessibility, antigenic index and flexibility of monkey B virus envelope protein gD was analysed and some potential peptide epitopes were forecasted.Then, the interactions between synthetic peptides and BV positive sera were detected by Enzyme-linked immunosorbent assay ( ELISA) .At last, The sensitivity and specificity of synthetic peptides was evaluated used 20 samples of Standard sera by ELISA.Result Seven epitopes were forecasted by Bioinformatics analysis. Four synthetic peptides, sequence as 46 LPPLEQKTD54 , 106 RGAPEATRSDA116 , 291 PELAPEERGTSRTPGD306 and 361 AVYLVRRRGR370 could be reacted with positive sera pool.The sensitivity of 4 synthetic peptides changed form 40% to 70% and the specificity were 100% for 4 synthetic peptides. Conclusion There are at least four linear epitope on B virus gD protein.
