Cloning and sequence analysis of dystonin(Dst) variant X1 in Cricetulusbarabensis and albino mutant Cricetulusbarabensis
10.3969/j.issn.1671.7856.2014.011.009
- VernacularTitle:黑线仓鼠及其白化突变系肌张力异常蛋白变体 X1基因的克隆与序列分析研究
- Author:
Simeng YI
;
Huifang LIU
;
Lin ZENG
;
Zhaozeng SUN
;
Xin WANG
- Publication Type:Journal Article
- Keywords:
Albino mutant Cricetulusbarabensisi;
Dystonin;
Cloning;
Sequence analysis
- From:
Chinese Journal of Comparative Medicine
2014;(11):45-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone and sequence the dystonin variant X1 gene of Cricetulusbarabensis and the albino mutant Cricetulusbarabensis so as to find out the difference of encoding arear of the muscular ribosome between Cricetulusbarabensis and the albino mutant Cricetulusbarabensis.Methods According to the same type of abnormal muscle tone protein of the mice and rats, we designed 6 pairs of primers, and got their cDNA genes from skins of the Cricetulusbarabensis and the albino mutant Cricetulusbarabensis by RT-PCR amplification, then cloned and sequenced. Results Sequence alignment showed 17 variances in coding areas, and 24 in amino acid, but no in key nucleic acid and protein.Conclusion The variances in coding areas will not lead to the albino, and its mechanism requires further investigation.