Comparison on rat and mice intestine slides’ quality fixed with three different fixatives and embeded by paraffin
10.3969/j.issn.1671.7856.2014.011.011
- VernacularTitle:三种固定液对大、小鼠肠道组织石蜡切片质量的影响
- Author:
Yuqiong ZAHO
;
Ruisheng LI
;
Hua CHEN
- Publication Type:Journal Article
- Keywords:
Fixative;
Intestine;
Slide;
HE staining
- From:
Chinese Journal of Comparative Medicine
2014;(11):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the rat and mice intestine slides quality fixed with three different fixatives and embeded by paraffin.Methods Three kinds of conventional fixatives were selected for the test, they are 10% neutral buffered formalin,Bouin’s solution and modified Davidson’ s solution.SD rat’ s and KM mice’ s intestinal tissues were fixed with the three different fixatives, then embeded, sliced and stained with HE as routine rut.To observe the slides quality under microscope.Results Sheding and necrosis of intestine villus epithelia were observed in slides made from formalin fixed intestines, which is speculated due to the fixations.The phenomenon was not observed in Bouin's solution and modified Davidson’ s solution fixed tissues.And the tissue structure were more fine and clear in them.Conclusions The rat and mice intestine slides made from tissues fixed by Bouin's solution and modified Davidson's solution are better than from the 10%neutral buffered formalin fixed ones.
