Establishement of phenobabital addiction rat model by modified drug admixed food method

VernacularTitle:改良掺食法建立苯巴比妥成瘾大鼠模型
Author: Bin HAN ; Guojun WANG ; Ping FU ; Yun YE
Publication Type:Journal Article
Keywords: drug-admixed food method; phenobarbital addiction; rat model
From: Chongqing Medicine 2014;(36):4924-4927
CountryChina
Language:Chinese
Abstract: Objective To establish the phenobarbital addiction rat model by the modified drug‐admixed‐food(DAF) method . Methods Rats were selected as the experimental animal .The modified DAF method was adopted to construct the model .The initial dose of phenobarbital 75 mg/kg was given mixed and the dase was gradually increased day by day ,which continued for 45 d .The es‐tablished model was verified by the precipitated withdrawal method and the natural withdrawal method for judging whether the model construction succeeding .Results The convulsion rate in the DAF precipitated withdrawal group was higher than that in the control group(P< 0 .01) ;the withdrawal symptoms scores in the DAF natural withdrawal group were higher than those in the con‐trol group(P< 0 .05) ;the relieving degree of body mass in the two withdrawal groups was greater than that in the control group (P< 0 .05) .The brain section in the mode group revealed obvious cellular apoptotic change .Conclusion DAF is simple and feasible for establishing the phenobarbital addiction rat model .