Effect of hedyotic diffusa willd injection on osteosarcoma MG-63 cells bax gene expression
10.3969/j.issn.1671-8348.2014.35.002
- VernacularTitle:白花蛇舌草对人骨肉瘤MG-63细胞Bax基因表达的影响
- Author:
Junli WANG
;
Kegong XIE
;
Ke HUANG
- Publication Type:Journal Article
- Keywords:
hedyotic diffusa willd;
osteosarcoma;
Bax gene
- From:
Chongqing Medicine
2014;(35):4708-4710
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hedyotic diffusa willd injection on osteosarcoma MG‐63 cells Bax gene expres‐sion .Methods MTT was be used to detect the inhibition rate of MG‐63 cells by hedyotic diffusa willd injection after 6 ,12 ,24 ,48 h certain concentration ,RT‐PCR was be used to detect the intracellular expression of Bax gene .Results Hedyotic diffusa willd injec‐tion can significantly inhibit the proliferation of MG‐63 cells ,as the concentration was 100 μL/mL ,Bax gene expression was signifi‐cantly increased over time(P<0 .05) .Conclusion Upregulating the expression of Bax gene by hedyotic diffusa willd injection can induced human osteosarcoma M G‐63 cells apoptosis to death .
