Species identification of partial sandflies from China with DNA barcoding

VernacularTitle:DNA条形码技术鉴定我国部分白蛉蛉种
Author: Zhengbin ZHOU ; Yi ZHANG ; Shan LV ; Wenqi SHI ; Changfa JIN ; Huaimin ZHU
Publication Type:Journal Article
Keywords: sandflies; COI; DNA barcoding
From: Chinese Journal of Zoonoses 2014;(12):1209-1213
CountryChina
Language:Chinese
Abstract: ABSTRACT:To explore the feasibility of DNA barcoding in the identification of sandflies ,the mitochondrial cytochrome c oxidase subunit I (COI) genes of 9 dominant species in China from three genera in the Phlebotominae were studied .The in‐traspecies and interspecies genetic distances were calculated using the Kimura‐2‐parameter model ,and the phylogenetic trees were reconstructed by neighbor‐joining (NJ) method .Results showed that all of sandflies species were successfully distinguish‐ed by the phylogenetic trees .The average intraspecific genetic distance (0 .8% ) was much less than the average interspecific ge‐netic distance (11 .2% ) .The study based on COI sequences indicates that the sandflies from China could be well distinguished with the commonly used COI barcodes ,which are potentially well utilized in sandflies species identification .