Determination of 17 Characteristic Ingredients of Plant Extracts in Hair Growth Cosmetics by Ultra High Performance Liquid Chromatography
10.11895/j.issn.0253_3820.140512
- VernacularTitle:超高效液相色谱法同时鉴定育发化妆品中17种植物提取物标识成分
- Author:
Jianhua TAN
;
Huiyong LI
;
Shaofeng XI
;
Changhong GUO
;
Jicai WANG
;
Xiaoting XIONG
;
Yanping XIAN
;
Xindong GUO
- Publication Type:Journal Article
- Keywords:
Ultra_high performance liquid chromatography;
Characteristic ingredients of plant extracts;
Hair growth cosmetics
- From:
Chinese Journal of Analytical Chemistry
2015;(1):110-114
- CountryChina
- Language:Chinese
-
Abstract:
A method was developed for the simultaneous determination of 17 characteristic ingredients of plant extracts, including paeoniflorin, hydroxysafflor yellow A, calycosin_7_glucoside ferulic acid, etc. , in hair growth cosmetics using ultra high performance liquid chromatography ( UPLC ) . Different cosmetic samples were extracted by ultrasonic_assisted extraction with the solvent of methanol/water (4∶1, V/V) solution. After demulsified by the addition of appropriate amount of NaCl and high speed centrifugation, the supernatant was transferred and analyzed with UPLC. The separation was conducted on a Waters reversed phase column of ACQUITY UPLC CSH C18(50 mm×2. 1 mm, 1. 7μm), and the mobile phases were methanol and the solution of 0. 05% phosphate in water. The detection was performed with a photodiode_array ( PDA) detector. The linear range was 0 . 2-25 mg/L with correlation coefficients higher than 0 . 999 . The limits of detection were within 0. 3-1. 5 mg/kg, and the limits of quantification were from 1. 0 to 4. 0 mg/kg. The average recoveries of 17 characteristic ingredients were within 93 . 5%-105 . 0%, with the intra_and inter_day precision ( n=6 ) less than 4. 6%. This method was simple, rapid, with good_repeatability, and had been applied to the analysis of real samples.