Effect comparison on intradermal injection and scratch inoculation of Brucella vaccine

VernacularTitle:布氏菌苗皮内与划痕接种的效果评价
Author: Cheng CHEN ; Dong WEI ; Kemei LI ; Lili FU ; Changjiang HUANG ; Guozhi WANG
Publication Type:Journal Article
Keywords: Brucella vaccine; humoral immunity; DTH
From: Chinese Journal of Zoonoses 2014;(12):1231-1233
CountryChina
Language:Chinese
Abstract: ABSTRACT:The guinea pigs were immunized by the Brucella vaccine through intradermal injections and the skin scratch respectively ,and then the immune effects of the two ways were evaluated .Serum samples were collected one month after the last injection and detected for the total IgG titer by interval ELISA .Cell‐mediated immune was evaluated by late‐onset hyper‐sensitivity .The guinea pigs were challenged with Brucella melitensis M5 ,and then were killed to isolated M5 from spleen of each guinea pig to compare the protective effects of two methods of immunization .The ELISA results showed that both of the two methods of immunization could induce strong humoral immune response ,and DTH response to Br‐PPD antigen were 100%in both methods .No significant difference in the immune protective effect of two methods was detected .Results of humoral im‐munity ,cellular immunity and protective effect showed the same effect by intradermal injections and skin scratches .