Fluorescein Mercury Combined with Optical Sensing Micro Sequential Injection Lab_on_Valve for Determination of H2 S in Intestinal Perfusate
10.11895/j.issn.0253_3820.140467
- VernacularTitle:基于荧光素汞荧光法结合光纤传感_微顺序注射_阀上实验室测定肠灌流液中H2 S
- Author:
Junfeng HOU
;
Xinxia LI
- Publication Type:Journal Article
- Keywords:
Fiber sensing;
Hydrogen sulfide;
Micro sequential injection lab_on_valve;
Fluorescein mercury;
Fluorescence quenching
- From:
Chinese Journal of Analytical Chemistry
2015;(1):132-136
- CountryChina
- Language:Chinese
-
Abstract:
A fluorescence quenching method was established for the determination of H2 S in intestinal perfusate by optical fiber sensing technology combined with micro sequential injection lab_on_valve (μSIA_lov). In the experiment, 100 μL of 0. 1 mol/L NaOH was used as the carrier, and 50 μL of 5. 0×10-5 mol/L fluorescein mercury and 50 μL of sample were selected for the determination. The detection flow rate at the flowcell was 25 μL/s. According to H2 S quenching fluorescence at 521 nm, the concentration of H2 S in the sample was determined. The detected concentration range of H2S was 5. 0×10-6-8. 0×10-5 mol/L, and the detection limit was 5. 4×10-7 mol/L. Detection result of H2S in intestinal perfusion was 3. 8×10-5 mol/L with 3. 1% RSD (n=3). This method can be used for the determination of H2S effectively in the samples, which lay the foundation for real_time online measurement of H2 S in biological samples.
