Synthesis and eukaryotic expressing of the gene encoding extracellular domains of gB protein B virus
10.3969/j.issn.1671.7856.2014.011.002
- VernacularTitle:猕猴B病毒gB蛋白胞外区基因片段的合成和真核表达
- Author:
Huifang LIU
;
Shufang SUN
;
Lin ZENG
;
Simeng YI
;
Ying YOU
;
Yunan MA
;
Junwen FAN
;
Zhaozeng SUN
;
Xin WANG
- Publication Type:Journal Article
- Keywords:
B virus;
gB protein;
Synthesis;
Eukaryotic expressing
- From:
Chinese Journal of Comparative Medicine
2014;(11):6-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To get the gene encoding extracellular domains of gB protein of B virus and analyze its expression in the eukaryocyte cell.Methods synthesizing gene fragment encoding extracellular domains of gB protein of B virus was by using synthesis gene, then digested with the restriction endonucleases BamHⅠand NotⅠand inserted into eukaryotic expressing vector pEGFP-N3.pEGFP-N3-GB合 was transfected into 293 cells.After protein extraction, the expression of gene was detcted by western blotting, and the cellular localization of the gene was analyzed by immunofluorescence and laser scanning confocal microscopy.Results pEGFP-N3-GB合were expressed in 293 cells and on the cell membrane.Conclusion eukaryotic expressing system can produce specific antigen recombination protein of B virus gB protein and express on the cell membrane.
