Discovery of four splicing isoforms of Beagle estrogen receptor β
10.3969/j.issn.1671.7856.2014.011.007
- VernacularTitle:比格犬雌激素受体β四个剪接异构体的发现
- Author:
Qin XU
;
Xiang DONG
;
Jianying LI
;
Yonghua XU
;
Xiumei REN
;
Yanbin ZHAO
;
Jieying BAI
;
Zhaozeng SUN
;
Lin ZENG
;
Zhongming HU
- Publication Type:Journal Article
- Keywords:
Beagles;
Estrogen receptorβ;
Splicing isoforms
- From:
Chinese Journal of Comparative Medicine
2014;(11):33-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the splicing isoforms of estrogen receptor βin the Beagle hypothalamic -pituitary -gonadal axis.Methods For ERβmRNA CDS sequence of eight exons, primers were designed confined to the CDS sequences of two sequential exons.Beagle hypothalamus, pituitary, ovary and uterus tissue cDNA were used as template, and corresponding sequences were amplified by PCR.PCR products were sequenced and aligned in the NCBI web site.The correct gene was then analyzed with DNAMAN comparative analysis software and handwork checking up, thus got the ERβsplicing isoforms of Beagle. Results Four beagle ER beta splicing isomers were obtained:exon 4 complete skipping ER βisomer (300 bp missing), two kind of Beagle ERβisoforms with partial exon 4 and partial exon 5 complicated missing (isoformⅠ334 bp missing and isoformⅡ265 bp missing), and exon 7 complete missing ERβsplicing isoforms (181 bp missing).Exon 4 complete skipping and exon 7 complete missing isomers had been obtained full length coding sequence, and the other two splicing isomers were partial coding sequence.Conclusion This project gained four ERβsplicing isomers of Beagle, and that will lay an important foundation for further study of their roles in the Beagle reproductive regulation mechanism.
