Simultaneous Detection of 25 Kinds of Veterinary Antibiotics in Soil, Manure and Water Samples Using Liquid Chromatography_Tandem Mass Spectrometry
10.11895/j.issn.0253_3820.140546
- VernacularTitle:超高效液相色谱/串联质谱法同时测定水、土壤及粪便中25种抗生素
- Author:
Xinyan GUO
;
Na WANG
;
Lijun HAO
;
Yong ZHI
;
Jing XU
;
Zanchang WANG
;
Zhengjun SHAN
- Publication Type:Journal Article
- Keywords:
Antibiotics;
Quinolones;
Sulfonamides;
Tetracyclines;
Macrolides;
Liquid chromatography_tandem mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2015;(1):13-20
- CountryChina
- Language:Chinese
-
Abstract:
A liquid chromatography_tandem mass spectrometric method was established for the determination of 25 kinds of veterinary antibiotics in manure, soil and water by ultrasonic_solid phase extraction. Purification efficiency of SAX cartridges for soil and manure samples extract and the procedure that manure samples defatted with hexane was also verified. The results showed that the average recoveries of the 25 target antibiotics were 50. 0%-121. 9%, the relative standard deviations (RSD) were 1. 1%-14. 71% (n=9), and the limits of detection (LOD) ranged from 0. 0002-0. 0560 μg/kg for soil and manure and ranged from 0. 002-0. 28 ng/L for water; After adding SAX cartridges, the matrix effects of manure samples reduced to 55%-120%, and soil samples to 75%-160%; after defatting by hexane extraction, the matrix effects of manure sample reduced to 55%-120%. This method has been employed to detect the veterinary antibiotics in environmental samples of livestock farm.
